Z Michalak, D Obari, M Ellis, M Thom, SM Sisodiya
Neurology 2017;88:1-11
Using immunohistochemistry, we
investigated the expression of 6 markers (CD163, human leukocyte
antigen–antigen D related, glial fibrillary acid protein, hypoxia-inducible factor-1a
[HIF-1a], immunoglobulin G, and albumin) in the hippocampus, amygdala, and medulla
in 58 postmortem cases: 28 SUDEP (definite and probable), 12 epilepsy controls,
and 18 nonepileptic sudden death controls. A semiquantitative measure of
immunoreactivity was scored for all markers used, and quantitative image
analysis was carried out for selected markers. Immunoreactivity was observed
for all markers used within all studied brain regions and groups.
Immunoreactivity for inflammatory reaction, BBB leakage, and HIF-1a in SUDEP
cases was not different from that seen in control groups. This study represents
a starting point to explore by immunohistochemistry the mechanisms underlying
SUDEP in human brain tissue. Our approach highlights the potential and
importance of considering immunohistochemical analysis to help identify
biomarkers of SUDEP. Our results suggest that with the markers used, there is
no clear immunohistochemical signature of SUDEP in human brain.
Free Full Text Article...
No comments:
Post a Comment